Part:BBa_K1319016

TEV protease cleavage site

This sequence codes for a TEV protease cleavage site.

ENLYFQ\S is the optimal cleavage site with the highest activity but the protease is also active to a greater or lesser extent on a range of substrates. The highest cleavage is of sequences closest to the consensus EXLYΦQ\φ where X is any residue, Φ is any large or medium hydrophobic amino acid and φ is any small hydrophobic amino acid.

Usage and Biology

A TEV protease is available codon optimised for E. coli with the part K1319004.

Contribution: HKUST 2022

Team HKUST 2022 uses the TEV protease cleavage site for their experiment on the characterization of their degradation system using TEV. By changing the position of the cleavage site and the LVA tag, HKUST Team 2022 achieve 2 purposes that is to increase output of a certain signal in a shorter period of time and reduce color mixing issue. For more information on how HKUST 2022 Team uses this cleavage site refer to the composite part BBa_K4225012 and BBa_K4225019

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]